Computational Prediction and Analysis of the NAPP – DR6 Interaction: Implications for Alzheimer's Research

Alzheimer's disease (AD) is a progressive neurodegenerative disorder that involves a devastating clinical course and lacks an effective treatment. A biochemical model for neuronal development, recently proposed by Nikolaev and co-workers, hinges on a novel protein-protein interaction between the death cell receptor six (DR6) ectodomain and an N-terminal fragment of amyloid precursor protein (NAPP). The model provides a coherent and satisfying framework for better understanding AD pathophysiology. Moreover, the DR6-NAPP interaction offers a tempting target for novel pharmacological intervention. Given all of this, we constructed a structural model of the DR6-NAPP interaction using the neurotrophin p75 receptor as a template. Importantly, crucial steps in the modeling pipeline were independently validated using the p75 receptor. The final docked model shows excellent agreement with a variety of biophysical and theoretical data sets. Particularly worth noting is the excellent observed agreement between the theoretically calculated DR6-NAPP binding free energy and the corresponding experimental quantity. In brief, the balance of the evidence suggests that the DR6-NAPP model proposed here should prove useful in future studies, modeling work and efforts aimed at structure-based drug design. Worcester Polytechnic Institute, Department of Chemistry and Biochemistry, 65 Prescott Drive, Worcester, Massachusetts 01609, email: [email protected] Sacred Heart University, Department of Chemistry, 5151 Park Ave, Fairfield, Connecticut 06825, email: [email protected] CMD Bioscience, LLC, 554 Boston Post Rd #318, Orange, Connecticut 06477, email: [email protected] Correspondence should be addressed to J.A. email: [email protected] N at ur e P re ce di ng s : h dl :1 01 01 /n pr e. 20 10 .4 54 5. 1 : P os te d 15 J un 2 01 0